Oxygen needs don’t stay static during fermentation. As biomass accumulates and metabolism shifts, the ideal air/O₂ mixture evolves, and matching it precisely can make the difference between a good batch and a “golden” one.
With ROSITA 2.0 Profile Programming, combined with dissolved oxygen cascade and direct MFC programming, you can automate air–oxygen adjustments over time, based on culture evolution and process stage.
In this HOW-TO Rosita 2.0 guide, we are demonstrating how to program an air/O₂ profile for a Pichia pastoris process, by gradually increasing O₂ enrichment from 24h to 96h, then returning to air-only, ensuring precision, reproducibility, and reliable optimisation from batch to batch.
