F3

Pilot to Industrial Bioreactor | Steam-in-Place Bioreactor

Multi useSIP

Stirred

Industrial

Premium SIP bioreactor for developing and scaling new bioprocesses, capable of enabling customisable engineering designs while being a highly tested and well-documented product that is difficult to find in industrial-sized environments. The third generation of a model with advanced and reliable technologies

What is it used for?

The F3 bioreactor by Bionet is a high-quality stainless steel bioreactor designed to meet the diverse needs of process development, scale-up, and commercial production. Ideal for microbiology and cell culturing, the F3 excels in supporting a range of applications across the biopharma, agriculture, and food sectors. Its robust design ensures reliable performance and scalability, making it the perfect choice for advancing your biotech processes from initial development through to large-scale production.

Customisation options

The F3 bioreactor by BIONET is engineered for both pilot and industrial environments, combining standardized SIP technology with a professional design and construction. Available in 50, 100, and 200-liter working volumes, it comes in distinct models optimized for microbial or cell culture applications. Its extensive range of ports and Plug&Play add-ons offer exceptional configurability and expandability, adapting seamlessly to evolving projects and multipurpose plants. This versatility makes it an ideal choice for advancing bioprocesses into industrial-scale production across biopharma, agriculture, and food sectors.

Key benefits

Ensure consistent performance with a robust product that has been thoroughly tested;
Rely on top-tier components and technologies, designed for precision and consistency in bioprocessing, ensuring quality, durability, and performance;
Ensure smooth fermentation operations through effective preventive maintenance with a recommended spare parts list for critical components and consumables;
Eliminate integration costs and risks with a single supplier for both upstream and downstream bioprocess equipment, ensuring a seamless process with Bionet’s integrated solutions.

Ensure efficient and contamination-free production at larger volumes with an SIP system;
Maintain consistent mixing, oxygen transfer, and temperature control from small-scale to large-scale production by preserving geometric similarity;
Guarantee optimal process scalability with standard solutions that are identical across all units (F0, F1, F2)

Adjust bioreactor to your specific need with more than 30 different add-ons for hundreds of possible combinations;
Adapt to evolving process requirements and technological advancements with a future-proof system featuring 6 free I/O signals, spare ports for new probes and actuators, and an expandable frame for seamless integration of add-ons;
Reduce upfront investment and allow easy expansion if needed with Plug&Play design philosophy.

Simplify operation and data access with software Marta, — designed with an intuitive user interface;
Facilitate maintenance and troubleshooting with ports, valves, and main components in an accessible 2D layout

Product features

A wide variety of stainless steel vessel types in different volumes

The vessels are available in a wide range of working volumes, from 20 to 200 liters and in different geometries, for microbial, cell culture and airlift solutions. Ports can be found on the lid, side and bottom.

A lid with easy maintenance and a variety of port configurations for different uses

The lid design offers a wide range of possibilities for operation due to available space for ports, which provide functional versatility for material and information exchanges. Easy cleaning, handling and maintenance are enabled thanks to a crane to lift the lid.

A powerful and precise bottom agitation module with easy maintenance

A precise motor to serve the complete vessel range and a wide range of rpm, with bottom agitation to free up space in the lid. Turbine options range from Rushton and Marine to other customized options upon demand. Discover more about it with Marta.

A premium quality pump configuration for specific needs, with advanced control

The addition module includes 3 peristaltic pumps for accurate and linear flow, enabling reliable scaling. Integration of optional additional pumps with variable speed allows for Batch, Fed-Batch or Continuous Processes. Integration with weight control is available. Discover more about it with Marta.

A reliable and precise gas module with flexible and advanced control options

The standard gas module in F3 units includes Air supply with rotameter, through sparger and overlay supply, as well as advanced control. Additional gas lines and associated control loops are available, with their feedback loops. MARTA enables advanced control.

An efficient integrated temperature control with ergonomic utilities management

The temperature module includes a jacket, combined with a primary circuit and advanced control with a feedback loop, in order to heat and cool automatically the contents of the bioreactor through the circulation of water from a cold water tank to the jacket. For control see Marta.

High quality instrumentation for advanced analysis

A set of standard sensors, and a range of available additional sensors enable reliable measurements, monitorization and data analysis as well as use of trigger values for feedback control of actuators.

High capacity condenser and pressure control for exhaust gas line

The equipment includes a shell and tube condenser, a manual needle and solenoid valves in exhaust gas to control pressure and moisture effectively. Automatic pressure control, exhaust gas filtering or heater can be included as optional Add-Ons.

Re-sterilizable sampling valve for simple sampling during operation

The sampling valve of the F3 equipment allows for multiple sampling during operation. The sampling solution is re-sterilizable and includes a manual steam valve and steam condensate outlet with steam trap.

Simple and safe SIP to facilitate operation

Simple and safe Sterilization in Place (SIP) to facilitate operation

There is an automatic sequence for the sterilization of the bioreactor, the rest of the sterilizations, such as transfer, addition, sampling and harvest lines when applicable, are performed manually. For control see Marta.

Includes a touch PC attached to the BCU and a license of MARTA software

MARTA has been developed on SIEMENS WINCC platform to guarantee advanced control, visualization and registry of data of the specific process, with the option of upgrading automation functions with additional modules. For more information see Marta.

Can be designed, built and qualified under GMP guidelines

Our GMP approach is structured so that design, construction and control can be adapted to your specific project and regulatory needs to allow the validation of your processes from standard to GMP.

Possibilities for future expansio - Plug and Play

Possibilities for future expansion – Plug&Play

The presence of accessible spare signals and wired connectors allows for future integration of additional instrumentation and actuators, and Bionet’s bSmart Modules in a Plug&Play fashion.