Bioreactors
search by:
Use
Mixing type
Sizes
Applications
Cell type
Client sector
Services
Bioreactors
search by:
Use
Mixing type
Sizes
Applications
Cell type
Client sector
Services
Our solutions by cell type
Pichia pastoris, a methylotrophic yeast species, has become a mainstay in research and industrial biotechnology. Known for its ability to produce high yields of recombinant proteins with proper folding and post-translational modifications, Pichia pastoris is a preferred host in many fermentation processes. Utilising a bioreactor system increases this capability, allowing precise control over environmental conditions and optimising protein expression.
Bionet is an ideal partner in Pichia Pastoris. We work together with you during the whole project lifecycle: from technology purchasing decisions to the development and optimization of the processes to industrial production.
Specialized expertise: Bionet is your strategic partner for the Pichia Pastoris fermentation process. Our team of experienced process specialists in collaboration with external industry peers hold more than 10 years of experience with this strain, with many achievements in the Pichia community, both in R&D and scale-up to thousand litres. This, together with the correct adaptation of our technologies to this cell line, aims to help you at any step of your work.
Advanced technology Bioreactors are central to the Pichia Pastoris fermentation process, providing a controlled environment to maximise growth and protein production. The integration of Pichia Pastoris fermentation with advanced bioreactor systems represents a powerful synergy for biotechnological production.
Discover how our solutions can improve your Pichia Pastoris fermentation process.
Achieve growth and productivity improvement by ensuring stable and accurate delivery of the gases. Bionet's gas modules allow a flexible selection of gas to be monitored, with optimised mass flow controller settings for the selected gas.
Incorporated triggers into the recipes for automotive transition control between the different growth stages of Pichia culture. Triggers are based on the changes in the values of the process parameters by the crop. It enables prompt reaction of automated systems to changes in critical parameters and therefore reduces contamination risk, downtime.
Without sufficient oxygen, the cells can switch to less efficient anaerobic metabolic pathways, leading to reduced growth rates and productivity. We solve this problem by incorporating a cascade dissolved oxygen control system that fine-tunes agitation and airflow, with proven PIDs, optimised for Pichia applications. In addition, pressure control regulation is included in the pilot scale as an alternative strategy to enrichment with pure O2.
View TFF System Pichia Pastoris secretes proteins into the extracellular medium, creating a challenge in separating cells and maximising product transfer. Our bioreactors address this issue by optimising pH filtration conditions based on the Isoelectric Point of the target protein, improving its passage through the membrane by leveraging its electrical charge. Additionally, we innovate with various diafiltration modes to refine the separation process, ensuring higher purity and concentration of the target proteins. These advanced techniques make our bioreactors the ideal solution for efficient and high-quality Pichia pastoris applications.
Celltainer is our single-use pilot bioreactor with patented two-dimensional rocking technology
Find all the additional modules that your bioreactor needs.
To establish the optimal moment of induction with methanol, it is important to know the state of the culture and the presence or complete depletion of limiting substrates to establish the optimal moment of induction with methanol. bBreath — Bionet's exhaust gas analyser provides real-time online information on the metabolic state of the culture. As an alternative to carbon source analysers as glycerol, glucose, or methanol sensors, exhaust gas analysers provide real-time online information on the metabolic state of the culture. It allows you to know the state of the culture and the presence or complete depletion of limiting substrates to establish the optimal moment of induction with methanol. It maximises the overall yield and thus improves high cell-density cultivation and expression of recombinant proteins.
With a simple cascade dissolved oxygen control system that fine-tunes agitation and airflow, with proven PIDs, optimised for Pichia applications, you will be able to increase dissolved oxygen. increased pressure in the fermentation vessels will lead to enhanced solubility of the oxygen in the culture medium and results in higher dissolved oxygen levels without the need for pure oxygen. It will also improve the oxygen transfer rate, sufficient oxygen to support metabolic activity and enhance the growth and productivity of cells.
