Bioreactors
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Bioreactors
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Use
Mixing type
Sizes
Applications
Cell type
Client sector
Services
Industrial TFF | Flexible membrane selection
Adaptability
In Bionet we are membrane agnostic, which allows us to integrate into our MS systems the membrane technology (tubular ceramic, hollow fibre, cassettes, spiral) that suits your application the best.
User-friendly design
All the components of the MS systems are easily accessible for maintenance tasks or manual operations involving a filtration process.
Consistent filtration rate
Temperature impacts both the filtration rate and the integrity of the product. To minimize process variability and ensure cell viability, it is crucial to perform filtration at a consistent and stable temperature. MS systems can be equipped with optional cooling and/or heating capabilities via jacketed recirculation vessels and/or dedicated in-line heat exchangers to maintain the desired temperature throughout the process.
Optimal quality and product integrity
Applications involving shear-sensitive products require careful handling to maintain their physical integrity and ensure cell viability throughout the filtration process. MS systems incorporate pumping technology that is specifically adapted to the nature of the product, ensuring gentle and effective processing.
Versatility in application-specific customisation
There are different operating modes depending on the variable that needs to be controlled (TMP, permeate flow, diafiltration volume, etc.). The choice of the operating mode depends mainly on the application (i.e. harvesting, clarification, concentration, diafiltration) for which the TFF is intended to be used. All MS systems can be designed to be operated at the mode set to the user's requirements.
Enhanced performance
Cross Flow Velocity or Shear Rate are the key parameters in a TFF system. Their monitoring and control ensure fouling delay and guarantee a stable filtration. MS systems are configured with the required flow transmitters to keep these parameters under control.
High yield and concentration factor
Applications involving biomass, cells, or retentate fractions (such as harvesting or biomolecule concentration) often require achieving high concentration factors, making the hold-up volume of the system a critical consideration. MS systems are designed to minimize hold-up volume in these scenarios, which may involve integrating the recirculation vessel within the same skid as the TFF system to optimize performance.
What is it used for?
The MS tangential flow filtration system combines extensive configurability with a range of standardized options designed to meet your specific downstream requirements. MS systems are the perfect solution for cell and lysate clarification, cell harvesting (especially important in the probiotics sector), or the concentration and diafiltration of proteins, peptides, enzymes, recombinant proteins, and monoclonal antibodies. They are also a good and safe choice for dehydrogenation purposes.
Customisation options
Bionet designs and builds TFF systems tailored to specific customer requirements, whether for unique design needs or large-scale operations. Our approach includes technology screening and process development and optimization to ensure that we select and refine the most suitable membrane configurations and technologies for your process. Our multi-purpose systems adapt to the membrane configuration and technology appropriate to your process, as well as other project needs, such as food and pharmaceutical regulatory considerations. We focus on delivering solutions that are customized to meet the precise needs of your project.
Bionet offers comprehensive support throughout the complete project life cycle, enabling process efficiency to meet evolving filtration needs. From initial technology assessment to detailed process optimization, we ensure robust project outcomes through proven methodologies and meticulous user requirements. We provide technology screening services to select the best possible solutions and process development services to design scalable processes.
MS is powered by built-in software MARTA — Bionet’s proprietary automation software, which allows unparalleled automation flexibility and tight control over the processes. It provides the user with ways to visualize, analyse and manage the data and take control of the processes. MS includes a touch screen attached to the BCU and a license of MARTA software. MARTA has been developed on the SIEMENS WINCC platform for advanced control, visualization and registry of data of the specific process. It guarantees you reliability and long-term maintenance of the solution.
Bionet’s TFF systems are designed to be cleaned in place (CIP) without the need for an extra external recirculation pump. Chemical addition can be performed using dedicated dosing pumps within the system or supplied externally by the customer. The system can be managed using MARTA software, which allows programming and saving cleaning cycle recipes for standardization and productivity. A SIP (Sterilise in Place) system can be installed upon request, sterilizing all parts of the TFF system without disassembly. This ensures a sterile environment, reducing contamination risk, essential for biopharmaceutical production.
Our TFF system has accurate flow and pressure controls to optimize filtration efficiency and avoid membrane damage. It makes MS a perfect application to work with any cells, even if they require the gentlest treatment.
Our GMP approach is structured so that design, construction and control can be adapted to your specific project and regulatory needs to allow the validation of your processes from standard to GMP.
It could be said that a TFF system has two core components: the membrane and the recirculation pump. The pump system is crucial and must be perfectly adapted to the nature of the product. Depending on the product's sensitivity to shear, Bionet’s MS systems can integrate various types of pumps, including centrifugal pumps and positive displacement pumps (such as lobular, diaphragm, and twin screws).
Success stories
Fully automated TFF system based on 2.5 m2 of ceramic membranes for 25X bacteria concentration. Incorporates a recirculation tank (600L WV). The feasibility of the technology was first validated at a pilot scale (50L) and then scaled up to 500L. The entire system, including membranes, can be cleaned (CIP) and sterilized (SIP) in place. Complies with FDA, cGMP and 21 CFR Part 11 requirements.
Success stories
Fully automated TFF system based on 3 m2 (expandable to 10 m2) of cassette membranes for toxin concentration purposes. Incorporates a recirculation tank (100L WV). The entire system can be chemically cleaned and sanitized in place. Complies with FDA, cGMP and 21 CFR Part 11 requirements.
Success stories
Fully automated TFF system based on 6.5 m2 of hollow fibre membranes for the clarification of bacterial lysates. Incorporates a recirculation tank (700L WV). The entire system can be cleaned in place (CIP). Sterilization in place (SIP) is also possible, except for membranes which are only chemically sanitized. Complies with FDA, cGMP and 21 CFR Part 11 requirements.
Specific services for TFF
Bionet can provide the bioreactors/fermenters, the downstream filtration systems and the ancillary equipment for comprehensive and integrated bioprocess solution.
Being vendor agnostic and with a strong experience in main filtration technologies (hollow fibre, cassettes, ceramic and spiral) Bionet can provide the exact solution you need for your validated process.
We provide technology screening services for selecting the best possible solution (including centrifugation) and process development services to design a scalable process.
Bionet can provide a process development service, or support your own, to generate the data and engineer and build the industrial-size systems which deliver the needed results in terms of product quality and recovery
We provide a project life cycle support with a strong focus on process and automation to help adapt your filtration to your changing needs.
Bionet offers two types of SIP (Sterilization In Place) systems to meet your needs. They produce complete SIP systems, where the entire circuit, including the membranes, is steam sterilized. Alternatively, they offer partial SIP systems, where the whole system is sterilized except for the membranes, which are bypassed during the sterilization process.
We design all our systems with the sensitivity of the product in mind. We carefully select membrane and pumping technologies to ensure they are suited to the nature of the product, minimizing the risk of product damage.
