HOW TO control substrate addition in ROSITA 2.0 using soft sensors and equation-based control in ROSITA 2.0

To optimise microbial growth and product formation, feeding strategies must adapt to the culture’s actual needs. But many key variables, like growth rate or biomass, can’t be measured directly in real time.

With ROSITA 2.0, you can calculate these values through soft-sensors and use them in equation-based control to automate feed rate adjustments based on what’s really happening in the bioreactor.

In this HOW-TO guide, we show how to configure virtual sensors to calculate parameters like biomass concentration and specific growth rate, and how to link those to dynamic feed control, resulting in more precise, optimised, and reproducible processes.