Recombinant protein expression in Pichia pastoris in a bioreactor via semi-continuous mode
Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production. Herein, we report the development of a semi-continuous process to improve the production of recombinant proteins in P. pastoris by its cultivation in a BIONET’s F1 benchtop 3-liter bioreactor equipped with a continuous process module (CPM).Six repetitive batches were conducted in a semi-continuous […]
Addition and Bioreactor Weight Controls
There are different fermentation modes in bioreactor systems: batch, fed-batch, and continuous mode. The batch mode is the simplest. The bioreactor is filled with the medium containing all the components that the organism requires to grow or produce determined biomolecules like a metabolite or protein. This fermentation mode has a production limitation: once the limiting […]
A Guide on Membrane Selection
The most common membrane modules used in the Biotech industry for Tangential Flow Filtration (TFF) are cassettes, hollow fiber and ceramic membranes. Each membrane type has certain characteristics (see Table 1) in terms of batch volume, sensibility to and with product type, and its own lifetime, that makes it more or less suitable for a specific broth or application.
Bioreactor Continuous Process
The Continuous Process in the context of a bioreactor operation mode is used to prolong the production phase of a culture during its growth in a steady-state (at a constant rate and in constant conditions) to achieve higher biomass and/or metabolites amounts. There are two main process control strategies that can be implemented in Continuous […]
5 steps into Scale-Up of Microbial Fermentation
Planning the jump into Industrial is a challenging experience that all successful bioprocesses and bioprocesists go through. With many factors having to be taken into account (at the biological, biochemical, mechanical levels among others), there is no other option than to follow a plan. What is the process information that cannot be dismissed? What process […]
Productivity enhancement of B. subtilis by cascade continuous fermentation
The majority of microbial fermentations are performed in batch or fedbatch mode, further increasing energy consumption and steps, limiting the productivity, conducting high costs. In some cases, these limitations are based on the equipment and technologies available [1]. The successful establishment of open production process based on continuous mode will lead to reducing the production […]
Bionet Continues Its Consolidation As A Leader In The Supply Of Complete Bioproduction Facilities
In Bionet’s trajectory a lot of the work and expertise has been developed around industrial scale projects, and 2017 has been a great year for this, with bioproduction plants put in place by Bionet for clients working in the monoclonal antibodies, animal vaccine and biocide production fields in three different European countries. As a representative example, Bionet has had the opportunity […]
Customization of agitation systems at its best
At BIONET, practical integration of knowledge derived from research as well as that gained from our clients’ experience happens on a continuous manner. This is vital to our evolution as a company and to the technological development and success of our products. An example of this is the effort that we deploy in adapting […]
Chemical cleaning of membranes
The chemical cleaning of the ceramic and/or hollow fiber membranes after a filtration stage must receive the attention it deserves. Having defined an effective cleaning protocol is just as important as having optimized filtration conditions when dealing with a tangential flow filtration process in your downstream. Moreover, I would dare to say, the latter is even more important. […]
