Production of the probiotic Lactobacillusrhamnosus GG under microaerophilicconditions in a single-use bioreactor using the Bionet Control System
Recent studies demonstrate that our microbiome can affect our health, not only with gastrointestinal diseases but also with other illnesses, such as asthma or psychological diseases (1,2). For this reason, the interest in the production of probiotics for therapeutic applications is increasing. The standards and requirements of the bioprocesses industry for high added value products, […]
Study of the effect of the substrate addition in Pichia pastoris cultures according to the evolution of exhaust gases as indicators of metabolism
The methylotrophic yeast Pichia pastoris is one of the most widely used recombinant protein expression systems, with applications from food to the pharmaceutical industry. It is capable of growing using methanol as the only carbon source, for which it uses alcohol oxidase (AOX) promoter, a strong inducer for recombinant protein expression. One of the critical […]
Isolation of Bacillussubtilis spores by Tangential Flow Filtration using the Bionet M1 system
In downstream processing (DSP), Tangential Flow Filtration (TFF) is one of the best options to separate different molecules and/or biomass according to particle size. Bacillus sp and related products are one of the bacteria most produced by fermentation technology since it has several applications in the biotechnology industry. The main goal in DSP operations with […]
Characterization of a Bacillus amyloliquefaciens fermentation for a fed-batch strategy using the Bionet’s bBreath exhaust gas analyzer
For aerobic fermentation processes, oxygen and carbon dioxide off–gas measurement allows a better characterization and a tool for process optimization, enabling new methods for the control process. In the present work, a fermentation process in fed-batch mode at bench–top scale for the production of the bacteria Bacillus amyloliquefaciens was performed using a Bionet F0–BABY and […]
How to use the microbial respiration in the aerobic fermentation process: OUR, CER and RQ determination with Bionet’s bBreath
During fermentation, many phenomena related to cell metabolism take place. In the case of aerobic fermentation, the parameters related to respiration are direct indicators of the state of the culture. In aerobic fermentation, a certain amount of O2 is needed to oxidize a relative quantity of carbon source into CO2 and H2O to obtain energy […]
Scale-up of E. Coli culture for optimal fed-batch strategy using exponential feeding
Escherichia coli is one of the most popular expression systems for the production of recombinant proteins. In the present study, a brief protocol to apply a fed-batch mode at lab scale with E.coli and the scale-up to 30 liters was performed. The high cell density of E.coli growth was achieved using a fed-batch mode in a Bionet F1 […]
Recombinant protein expression in Pichia pastoris in a bioreactor via semi-continuous mode
Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production. Herein, we report the development of a semi-continuous process to improve the production of recombinant proteins in P. pastoris by its cultivation in a BIONET’s F1 benchtop 3-liter bioreactor equipped with a continuous process module (CPM).Six repetitive batches were conducted in a semi-continuous […]
Addition and Bioreactor Weight Controls
There are different fermentation modes in bioreactor systems: batch, fed-batch, and continuous mode. The batch mode is the simplest. The bioreactor is filled with the medium containing all the components that the organism requires to grow or produce determined biomolecules like a metabolite or protein. This fermentation mode has a production limitation: once the limiting […]
A Guide on Membrane Selection
The most common membrane modules used in the Biotech industry for Tangential Flow Filtration (TFF) are cassettes, hollow fiber and ceramic membranes. Each membrane type has certain characteristics (see Table 1) in terms of batch volume, sensibility to and with product type, and its own lifetime, that makes it more or less suitable for a specific broth or application.
