In fed-batch fermentations, it’s common to add different substrates at different times, each with its own purpose and ideal flow profile. But handling this manually often leads to inconsistency, mistimed additions, and irreproducible results.
With ROSITA 2.0, you can combine feeding tools (constant, lineal, exponential) with the profile programming tool to automate the addition of multiple substrates using independent, variable-speed pumps.
In this guide, we show how to control the addition of two carbon sources (glycerol and methanol) during a Pichia pastoris process. By programming precise timings and feed rates, you adapt your control strategy to each phase, from growth to induction.
You can achieve more precision, fewer errors, and better reproducibility across batches without manual intervention.
