Categoría: Scientific Academy

The bioprocess industry is at the moment a dynamic one, with many new bioprocesses coming in as well as others being at the spotlight for optimization. At Bionet we are constantly exploring new ways in which the bioprocess applications can find their highest potential, and as good scientists we need to support these words with data. By clicking in this section a selection of Scientific Publications by Bionet’s application specialists will be selected for you.

Production in semi-continuousmode with the yeast Pichia pastorisusing BIONET’s F1 bioreactor

The yeast Pichia pastoris, has been successfully used as an efficientsystem to produce heterologous proteins [1]. Herein, we report thedevelopment of a semi-continuous process to improve the productionof recombinant proteins in P. pastoris in BIONET’s F1 bench-top3-liter bioreactor, equipped with a continuous process module (CPM).Six repetitive batches were conducted in a semi-continuous processby replacing 2/3 […]

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Scale-up of a bioprocess forbio-polymers productionusing animal by-products.

The production model of circular economy is becoming a focus ofattention in our society. One of the solutions proposed by this modelis waste revalorization. Most of the waste revalorization processesare based in the biotransformation of these waste materials intonew valuable ones. In previous works, a fermentation process thataimed to use slaughterhouse waste as a substrate […]

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Production of the probiotic Lactobacillusrhamnosus GG under microaerophilicconditions in a single-use bioreactorusing the Bionet Control System

Recent studies demonstrate that our microbiome can affect our health, notonly with gastrointestinal diseases but also with other illnesses, such as asthma or psychological diseases (1,2). For this reason, the interest in the production of probiotics for therapeutic applications is increasing. The standardsand requirements of the bioprocesses industry for high added value products,such as probiotics, […]

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Study of the effectof the substrate additionin Pichia pastoriscultures according tothe evolution of exhaustgases as indicatorsof metabolism

The methylotrophic yeast Pichia pastoris is one of the most widely used recombinant protein expression systems, withapplications from food to the pharmaceutical industry. It is capable of growing using methanol as the only carbonsource, for which it uses alcohol oxidase (AOX) promoter, a strong inducer for recombinant protein expression. One ofthe critical aspects of methanol […]

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Isolation of Bacillussubtilis spores byTangential FlowFiltration using theBionet M1 system

In downstream processing (DSP), Tangential Flow Filtration (TFF) is one of the best optionsto separate different molecules and/or biomass according to particle size. Bacillus sp andrelated products are one of the bacteria most produced by fermentation technology since ithas several applications in the biotechnology industry. The main goal in DSP operations withBacillus sp processes is […]

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Characterization of a Bacillus amyloliquefaciens fermentation for a fed-batch strategy using the Bionet’s bBreath exhaust gas analyzer

For aerobic fermentation processes, oxygen and carbon dioxide off–gas measurementallows a better characterization and a tool for process optimization, enabling new methodsfor the control process. In the present work, a fermentation process in fed-batch modeat bench–top scale for the production of the bacteria Bacillus amyloliquefaciens wasperformed using a Bionet F0–BABY and the exhaust gas analyzer […]

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Scale-up of E. Coli culture for optimal fed-batch strategy using exponential feeding

Escherichia coli is one of the most popular expression systems for the production of recombinant proteins. In the present study, a brief protocol to apply a fed-batch mode at lab scale with E.coli and the scale-up to 30 liters was performed. The high cell density of E.coli growth was achieved using a fed-batch mode in […]

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Recombinant protein expression in Pichia pastoris in a bioreactor via semi-continuous mode

Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production. Herein, we report the development of a semi-continuous process to improve the production of recombinant proteins in P. pastoris by its cultivation in a BIONET’s F1 benchtop 3-liter bioreactor equipped with a continuous process module (CPM).Six repetitive batches were conducted […]

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Monoclonal Antibody Production

The core of the technological infrastructure required for biopharmaceutical manufacturing is the bioreactor, used in all stages from R&D to biomanufacturing. The F0-BABY bioreactor line by BIONET is comprised of robust and user-friendly laboratory bioreactor models that can be configured for different cell expression systems like microbial cultures or cell cultures. In the present work, […]

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Recombinant Protein Expression in P. pastoris in a bioreactor via a semicontinuous mode

Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production.Herein, we report the development of a semicontinuous process to improve the production of recombinant proteins in P.pastoris by its cultivation in a BIONET’ s F1 benchtop bioreactor of 3 L equipped with a continuous process module(CPM). Six repetitive batches were […]

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