Categoría: Scientific Academy

The bioprocess industry is at the moment a dynamic one, with many new bioprocesses coming in as well as others being at the spotlight for optimization. At Bionet we are constantly exploring new ways in which the bioprocess applications can find their highest potential, and as good scientists we need to support these words with data. By clicking in this section a selection of Scientific Publications by Bionet’s application specialists will be selected for you.

Characterization of a Bacillus amyloliquefaciens fermentation for a fed-batch strategy using the Bionet’s bBreath exhaust gas analyzer

For aerobic fermentation processes, oxygen and carbon dioxide off–gas measurementallows a better characterization and a tool for process optimization, enabling new methodsfor the control process. In the present work, a fermentation process in fed-batch modeat bench–top scale for the production of the bacteria Bacillus amyloliquefaciens wasperformed using a Bionet F0–BABY and the exhaust gas analyzer […]

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Scale-up of E. Coli culture for optimal fed-batch strategy using exponential feeding

Escherichia coli is one of the most popular expression systems for the production of recombinant proteins. In the present study, a brief protocol to apply a fed-batch mode at lab scale with E.coli and the scale-up to 30 liters was performed. The high cell density of E.coli growth was achieved using a fed-batch mode in […]

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Recombinant protein expression in Pichia pastoris in a bioreactor via semi-continuous mode

Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production. Herein, we report the development of a semi-continuous process to improve the production of recombinant proteins in P. pastoris by its cultivation in a BIONET’s F1 benchtop 3-liter bioreactor equipped with a continuous process module (CPM).Six repetitive batches were conducted […]

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Monoclonal Antibody Production

The core of the technological infrastructure required for biopharmaceutical manufacturing is the bioreactor, used in all stages from R&D to biomanufacturing. The F0-BABY bioreactor line by BIONET is comprised of robust and user-friendly laboratory bioreactor models that can be configured for different cell expression systems like microbial cultures or cell cultures. In the present work, […]

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Recombinant Protein Expression in P. pastoris in a bioreactor via a semicontinuous mode

Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production.Herein, we report the development of a semicontinuous process to improve the production of recombinant proteins in P.pastoris by its cultivation in a BIONET’ s F1 benchtop bioreactor of 3 L equipped with a continuous process module(CPM). Six repetitive batches were […]

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Productivity enhancement of B. subtilis by cascade continuous fermentation

The majority of microbial fermentations are performed in batch or fedbatch mode, further increasing energy consumption and steps, limiting the productivity, conducting high costs. In some cases, these limitations are based on the equipment and technologies available [1]. The successful establishment of open production process based on continuous mode will lead to reducing the production […]

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Effect of fermentation defoaming agents on TFF

Tangential flow filtration (TFF), also known as cross flow filtration (CFF), comprises a rising technology in the last decades for the dewatering, isolation, and concentration of products of interest (e.g. biomolecules, metabolites and cells) from bioprocesses. Tangential Flow fitlration consists on the passage of the product feed in parallel to the surface of the filtering […]

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