Bioreactor Continuous Process
The Continuous Process in the context of a bioreactor operation mode is used to prolong the production phase of a culture during its growth in a steady-state (at a constant rate and in constant conditions) to achieve higher biomass and/or metabolites amounts. There are two main process control strategies that can be implemented in Continuous […]
5 steps into Scale-Up of Microbial Fermentation
Planning the jump into Industrial is a challenging experience that all successful bioprocesses and bioprocesists go through. With many factors having to be taken into account (at the biological, biochemical, mechanical levels among others), there is no other option than to follow a plan. What is the process information that cannot be dismissed? What process […]
Never give anything for granted when it comes to cultivating mammalian cells!
It is widely agreed that the most important quality factor of a bioreactor is its capacity to keep its inside sterile throughout the whole process and despite the number of operational human interactions that will take part during such period, including sampling and harvesting steps. Therefore, it is widely assumed that a bioreactor will do its part in meeting […]
Effect of fermentation defoaming agents on TFF
Tangential flow filtration (TFF), also known as cross flow filtration (CFF), comprises a rising technology in the last decades for the dewatering, isolation, and concentration of products of interest (e.g. biomolecules, metabolites and cells) from bioprocesses. Tangential Flow fitlration consists on the passage of the product feed in parallel to the surface of the filtering […]
Productivity enhancement of B. subtilis by cascade continuous fermentation
The majority of microbial fermentations are performed in batch or fedbatch mode, further increasing energy consumption and steps, limiting the productivity, conducting high costs. In some cases, these limitations are based on the equipment and technologies available [1]. The successful establishment of open production process based on continuous mode will lead to reducing the production […]
Bionet Continues Its Consolidation As A Leader In The Supply Of Complete Bioproduction Facilities
In Bionet’s trajectory a lot of the work and expertise has been developed around industrial scale projects, and 2017 has been a great year for this, with bioproduction plants put in place by Bionet for clients working in the monoclonal antibodies, animal vaccine and biocide production fields in three different European countries. As a representative example, Bionet has had the opportunity […]
Customization of agitation systems at its best
At BIONET, practical integration of knowledge derived from research as well as that gained from our clients’ experience happens on a continuous manner. This is vital to our evolution as a company and to the technological development and success of our products. An example of this is the effort that we deploy in adapting […]
Chemical cleaning of membranes
The chemical cleaning of the ceramic and/or hollow fiber membranes after a filtration stage must receive the attention it deserves. Having defined an effective cleaning protocol is just as important as having optimized filtration conditions when dealing with a tangential flow filtration process in your downstream. Moreover, I would dare to say, the latter is even more important. […]
Recombinant Protein Expression in P. pastoris in a bioreactor via a semicontinuous mode
Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production.Herein, we report the development of a semicontinuous process to improve the production of recombinant proteins in P.pastoris by its cultivation in a BIONET’ s F1 benchtop bioreactor of 3 L equipped with a continuous process module(CPM). Six repetitive batches were conducted in a […]
