Our solutions by cell type

E. Coli Bioprocessing

Maximise your E. coli fermentation with Bionet

Escherichia coli (E. coli) is a versatile and widely utilized microorganism in industrial biotechnology, renowned for its high transformation efficiency, rapid growth, and robust protein expression capabilities. Its ability to be easily genetically engineered has made it invaluable in producing a wide range of specialized strains that enhance protein folding, transcriptional regulation, and even the production of complex eukaryotic proteins. These characteristics make E. coli essential for applications in pharmaceuticals, enzyme production, and synthetic biology, where it is used to produce therapeutic proteins, industrial enzymes, and other valuable bioproducts.

Why Bionet?

Bionet is an ideal partner in E.coli. We work together with you during the whole project lifecycle: from technology purchasing decisions to the development and optimization of the processes to industrial production.

With extensive expertise in E.coli bioprocessing, Bionet’s team of specialists is committed to supporting you at every stage. From selecting the right strains to optimizing processes and scaling up to industrial production, our experts provide tailored guidance to ensure your project’s success. We leverage our deep knowledge and experience to help you achieve the highest levels of efficiency and productivity, making Bionet the perfect partner for your E. coli fermentation needs. More about Bionet’s upstream and downstream services.

Bionet’s bioreactors and TFF systems are designed to optimise E. coli fermentation processes. Our bioreactors offer precise control over critical parameters like temperature, pH, and oxygen levels, ensuring optimal growth conditions and maximizing recombinant protein yield. Our TFF systems efficiently separate and concentrate target proteins, improving downstream processing. Discover, how our solutions can contribute to your success and improve the efficiency and scalability of your E. coli fermentation projects.

Complete E. Coli Solutions

Enhance scalability

Maintains stable temperature conditions across different scales, from lab to production, ensuring reliable E. coli fermentation results with PID-controlled temperature regulation;
Adapt to varying production needs by efficiently managing oxygen and other gases supporting scalability from small-scale to large-scale E. coli cultures with flexible gas control;
Achieve seamless scalability with the Celltainer's flexible working volume range in a single bag;
Get real-time metabolic data that supports consistent process performance across various scales with the bBreath exhaust gas analyzer.

Optimise operational cost

Ensure precise substrate dosing and reduce resource overuse with gravimetric substrate addition;
Optimise nutrient addition and induction phases based on real-time data, lowering costs in E. coli processes with automated induction and feeding control.
Reduce energy consumption and operational costs by efficiently managing gas usage with optimized gas control.

Improve productivity

Enhance E. coli growth and product output with automated fed-batch optimization by improving nutrient addition based on real-time CO2 evolution and respiration coefficients;
Maintain optimal conditions through PID-regulated temperature and cascade DO control, improving productivity in E. coli fermentation;
Automate induction based on real-time optical density measurements, maximizing E. coli yield with integrated OD probes.

Automate the process

Automate transitions between culture phases based on real-time data, reducing manual adjustments with integrated into Bionet's software Rosita 2.0 recipe programming;
Ensure precise gravimetric dosing of substrates and automate nutrient delivery by integrating the bScale with addition pump control;
Automate adjustments and reduce manual oversight with continuous feedback by real-time metabolic monitoring with a bBreath analyzer.

Concrete solutions by our technologies

PID-controlled temperature regulation to maintain optimal temperature control

Temperature variations can negatively impact E. coli growth and protein production. Our PID-controlled temperature regulation maintains precise and stable temperature conditions throughout the fermentation process. This ensures that temperature is consistently optimal, enhancing fermentation reliability and yield.

Flexible gas and mass flow controllers to optimise gas management

Inconsistent gas levels can lead to poor aerobic conditions and reduced E. coli growth. Our flexible gas control system, equipped with a gas module and mass flow controllers, allows for precise adjustment of oxygen and other gases. This ensures optimal gas conditions, supporting efficient scalability from small to large-scale fermentations.

bScale to enhance precision with gravimetric substrate addition

Inaccurate substrate dosing can lead to inefficiencies and inconsistent E. coli growth. Our gravimetric substrate addition system, integrated with the bScale communication system, precisely measures substrate weight instead of volume. This reduces dosing errors and optimizes resource utilization, improving overall fermentation precision.

Integrated optical density probes for automate induction

Manual induction timing can lead to suboptimal E. coli yield and inconsistent results. Our integrated optical density probes continuously measure the optical density of the culture. This data triggers automatic induction at the optimal moment, ensuring better yield and more efficient fermentation.

Software-integrated recipe programming

Frequent manual adjustments during fermentation can lead to inefficiencies and errors. Our software-integrated recipe programming automates transitions between fermentation phases based on real-time data like optical density or CO2 levels. This streamlines the process and ensures precise control, reducing the need for manual intervention.

bBreath Exhaust Gas Analyzer to improve process control by monitoring metabolic data

Monitoring the metabolic state of E. coli cultures manually can be time-consuming and imprecise. Our bBreath exhaust gas analyzer provides real-time data on CO2 evolution and respiration coefficients. This feature enables accurate adjustments to nutrient feeding and other process parameters, optimizing fermentation efficiency.