Production of the probiotic Lactobacillusrhamnosus GG under microaerophilicconditions in a single-use bioreactorusing the Bionet Control System

 In Scientific Academy

Recent studies demonstrate that our microbiome can affect our health, not
only with gastrointestinal diseases but also with other illnesses, such as asthma or psychological diseases (1,2). For this reason, the interest in the production of probiotics for therapeutic applications is increasing. The standards
and requirements of the bioprocesses industry for high added value products,
such as probiotics, are increasingly demanding, including reducing the
preparation and cleaning times and the contamination risks, as well as a high
level of automation and versatile and flexible control systems, to be adapted
to different needs and requirements of processes. The probiotic Lactobacillus
rhamnosus GG, which has raised increasing interest for its beneficial effects
in human health (3-6), is a Gram-positive, non-sporeforming, facultatively
anaerobic or microaerophilic bacteria. Herein, the production of this bacteria
is presented, using a 3-liter single-use bioreactor (SUB) coupled with the
Bionet Control System for bench-top bioreactors, and compared to a classical glass vessel to demonstrate the feasibility of using the Bionet Control
System for control and monitorization of a fermentation process with a
SUB. The profiles of pH and DO under microaerobic conditions with a reverse cascade were similar in both systems, with a good control temperature
using only a heating blanket

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