Downstream Bioprocessing

M1 Labscale Tangential Flow Filtration System

benchtop TFF system

Your flexible tangential flow filtration (TFF) system for the concentration/clarification of your bioprocess’s biomolecules and biomass at lab and pilot use and in small production.

IT’S TIME TO DEVELOP YOUR DOWNSTREAM AND CHOOSE A SUITABLE LABSCALE TFF SYSTEM!

Don’t hesitate in jumping ahead and testing Tangential (Cross) Flow Filtration (TFF) for your bioprocess downstream. TFF could well be the most suitable technology for this, and it is very important you start testing your process performance as soon as you have a feedstock to try on.

The M1 unit is a unique solution for all those seeking a compact system for concentration and/or clarification of the biomass and/or biomolecules from their microbial fermentation/culturing processes at lab scale.

WHY IS M1 AN UNIQUE LABSCALE SYSTEM FOR TECHNOLOGY SCREENING, OPTIMIZATION AND SCALE-UP ACTIVITIES?

There are a number of product characteristics and technology configurations that have an impact in the potential of TFF to your process and its scalability to larger scales.

The M1 offers the flexibility (in the form of modularity and membrane selection) required for creating the right configuration for your filtration system.

Moreover, it provides you with the right technological components (e.g. recirculation pump) and process flow for your process scalability. Meaning you can be confident that your results can be transferred to a production scale.

Finally, the combination of a IOT-based architecture and our ROSITA software solutions renders your process consistent and reproducible, and provides the data for a complete test analysis.

M1 is the right tool for:

  • Technology selection (membrane technology and materials).
  • Selection of optimum pore size.
  • Calculation of achievable concentration factor.
  • Generation of filtrability curves.
  • Cleaning procedures.
  • Evaluation of the impact of the operative parameters (e.g. cross-flow velocity, pressyre and temperature) regarding yields, flows an dproduct quality.
  • Small productions.

THE FLEXIBILITY YOUR DOWNSTREAM NEEDS

The M1 allows you to select the most optimum membrane technology and use a complete range of pore sizes, surface areas and channel sizes, so that you find the right solution; without the limitations of membrane vendor systems. This ramge of options, briefly stated here, provides an enourmous flexibility.

    • Three types of membranes, Cassettes, Ceramic and Hollow Fibre, from main leading vendors, which can be interchangeably integrated and tested in the M1. This allows for un-parallel capabilities for comparing membranes using identical process conditions and data registration for analysis.
    • These membranes are available, in tirn, in a number of configurations:
    • 21 different pore sizes in the microfiltration, ultrafiltration and spaces (from 1.4 µm in the microfiltration space to less than 1kDa in the ultrafiltration space).
    • Different number of channels (in ceramic) and thereby membrane surface area available.

THE SCALABILITY FOR YOUR PROCESS FUTURE

bFlow: a Smart feeding pump

The bFlow is the smart pump used as a feeding pump in the M1. It is the core of our M1 series and part of our Bionet bSmart family of lab process modules. It is an exceptional pump with capabilities beyond standard lab pumps.

What makes it so exceptional?

  • A unique range of flows and pressure. From 800 to 10,000 ml per minute and from 0 to 6 barg working pressure. All driven by a servomotor for precise control of rpms.
  • The right pump technology. The multilobe pump technology guarantees your lab results are truly valid for scale-up and provides extraordinary gentleness for sensible biomass and biomolecules.
  • Connected and smart. The bFlow comes with ready connections to multiple sensors and data logging for batch data recording for a later analysis. Beyond that you can automate some process parameters.
  • Double interface. You can control the pump through a physical control panel with buttons and display or through a web interface through a wireless connection with ROSITA SW.
Software interface for TFF data logging and some automatic controls

AUTOMATION AS A FRIEND

The M1 is a manual unit with some automatic controls and full data registration of system and process parameters.

This data come from actuators or instruments as well as a result of calculations which can be made based on the registered data and that allows for further data logging and additional control strategies.

    • Cross flow rate. Or recirculation rate or retentate flow can be monitored and controlled with the addition of a Flowmeter (Optional) with a control loop with the pump driver.
    • Filterability. Permeate flux (in l/h) can be monitored with a connected scale (Optional).
    • Product temperature. With a TT on the vessel.
    • Pressure. Pressure is measured in 2 points as standard (feed and permeate) and one optional (permeate) allowing for calculation of Transmembrane Pressure and other critical process parameters.

SUPPORT AS THE CORE OF OUR STRATEGY

With the aim to reduce our clients’ upfront technology investment at early phases and/or to help them obtain the highest productivity with their processes, all our technologies are accompanied by advanced packages of Process Services.

At the stages of evaluating TFF as a potentially suitable Downstream technology for your process, and of selecting the right TFF system configuration for your process, here you have a link to the Downstream Process Services that could become relevant:

    • Downstream Technology Screening
    • Downstream Proof-of-Concept Trials
    • Downstream Optimization Services

M1 TECHNICAL SPECIFICATIONS

APPLICATION DETAILS
APPLICATION DETAILS
Applications
  • Microfiltration, Ultrafiltration
Batch volume
  • Up to 20L (assuming a good filtrability)
DESIGN DETAILS
DESIGN DETAILS
Operation footprint (H x W x D)
  • 540 x 650 x 600 mm
Vessel working volume
  • 3 L (other volumes available)
Vessel type
  • Single wall (jacketed vessel available)
Vessel material
  • Borosilicate Glass
Dead volume (ml)
  • 200
MEMBRANES
MEMBRANES
Membrane vendor
  • Neutral
Membrane type
  • Reusable and single-use
Membrane types
  • Ceramic, Hollow Fiber and Cassettes
MEMBRANES Pore Size (for MF)
MEMBRANES Pore Size (for MF)
Ceramic (micrometers)
  • 0.05, 0.1, 0.14, 0.2, 0.4, 0.45, 0.5, 0.8, 1.2, 1.4
Hollow Fiber (micrometers)
  • 0.1, 0.2, 0.45, 0.65
Cassette (micrometers)
  • 0.1, 0.2, 0.45, 0.65
MEMBRANES Pore Size (for UF)
MEMBRANES Pore Size (for UF)
Ceramic
  • 1, 5, 10, 20, 50, 100, 150, 300 kD
    20, 50 nm
Hollow Fiber
  • 1, 3, 5, 10, 30, 50, 70, 100, 300, 500, 750 kD
Cassette
  • 1, 3, 5, 8, 10, 30, 50, 70, 100, 200, 300, 500, 1000 kD
MEMBRANES Surface área
MEMBRANES Surface área
Ceramic
  • up to 0,01 m2
Hollow Fiber
  • up to 0,079 m2
Cassette
  • up to 0,5 m2
bFlow (recirculation pump)
bFlow (recirculation pump)
Material
  • SS 316L Rotor and casing, EPDM Elastomers
Surface finishes
  • Inner surfaces finished to Ra 0.6μm
Motor
  • Servo-motor drive for maximized flow range with Integrated servo controller
Operation details
  • Fully drainable CIP compatible design
Flow range
  • 0.8-10 L/min
Pressure range
  • 0 – 6 barg
PROCESS CONTROL
PROCESS CONTROL
PROCESS CONTROL Manual/Non-feedback Automated Feedback Automated (a.k.a control loop) Data log Description
bFlow speed (rpm) • ◦ • Basic: configurable pump “rpm” and pump data log Optional: feedback-automatic control and flow data log )if recirculation flow meter is installed)
Recirculation flow • ◦ ◦ Basic: configurable pump “rpm” and pump data log Optional: feedback-automatic control and flow data log )if recirculation flow meter is installed)
TMP (Transmembrane Pressure) • – ◦ Basic: manual control of retentate manual valve Optional: calculation and data log (if permeate pressure is installed)
Permeate pressure ◦ – • Optional: indirect manual control (if manual valve is installed); data log (if permeate sensor is installed)
Feed Pressure – – • Basic: indirect control; data log (since sensor is installed)
Retentate Pressure – – • Basic: manual control (since retentate manual valve is included); and data log (since sensor is installed)
Permeate Flow – – • Optional: manual control (if manual valve is installed); data log (if scale is installed)
Product temperature – – • Basic: data log (since TT is included)

TECHNICAL SPECIFICATIONS

MODEL M1

commercial claims and positioning
commercial claims and positioning
Applications
  • Microfiltration, Ultrafiltration
Brief description
  • Ideal for concentration/purification of biomolecules (in the food, agriculture, industrial biotech, and biopharma sectors) of small volumes at R&D scale and development, optimization and scale-up activities
dimensions
dimensions
Operation footprint (h x w x l)
  • 500 x 600 x 700 mm
vessel and volumes
vessel and volumes
Concentration factor
  • -
Dead volume (ml)
  • 300
Jacketed
  • Included by default
Recomended batch volume
  • -
Vessels working volume
  • 4.5 L
membranes spiral wound
membranes spiral wound
Effective length
  • -
Pore size
  • -
Pressure
  • -
Surface area
  • -
Temperature
  • -
membranes hollow fiber
membranes hollow fiber
Effective length
  • 20 cm
Pore size
  • 0.05-0.65 μm (MF)
  • 1-750 kDa (UF)
Pressure
  • 50º
Surface area
  • 0.047-0.1 m2
Temperature
  • 2 barg
membranes ceramic
membranes ceramic
Effective length
  • 20 cm
Pore size
  • 0.1-1.4 μm (MF)
  • 1(UUF)-10-750 kDa
Pressure
  • 80º
Surface area
  • 0.0047-0.0132 m2
Temperature
  • 0.5-2 barg (MF)
  • 0.5-5 barg (UF)
recirculation pump
recirculation pump
Motor
  • 750W, 2200 rpm (at 50Hz)
agitation
agitation
Agitator
  • -
Speed (rpm)
  • -
Turbines
  • Optional
temperature actuators
temperature actuators
Cooling
  • Opening of valves for renewal of direct chilled water supply from external chiller to jacket water circuit
Heating
  • Electrical resistance within water circuit
instrumentation
instrumentation
Conductivity
  • Optional
Feed flow indicator
  • -
Level transmitter
  • -
Load cell/Permeate scale
  • Optional
Permeate flow indicator
  • -
Pressure indicator at membrane module entry
  • Included by default
Pressure indicator at membrane module exit (retentate return)
  • Included by default
Pressure indicator at permeate return
  • -
Pressure transducers (to allow automation and alarms)
  • Optional
Tank temperature
  • Included by default
ph
  • Optional
process parameters general
process parameters general
Fluid Pressure
  • 6 barg (ceramic)
  • 2 barg (hollow fiber)
Minimum working volume
  • 100-200 ml
Sterilization
  • -
Temperature
  • 80º (ceramic)
  • 50º (hollow fiber)
process parameters manual
process parameters manual
Feed flow rate (rpm)
  • Included by default (via pump frequency variator)
Process finalization
  • Included by default (via manual stoppage of pump)
TMP
  • Included by default (via back-pressure valve)
process parameters automatic
process parameters automatic
Permeate Back Pressure
  • Optional (via Back Pressure Valve)
Safety pressure
  • -
TMP
  • Optional (via Back Pressure Valve)
Temperature (via automatic valves)
  • Optional
process parameters calculations
process parameters calculations
Concentration Factor
  • -
Filtration flux rate
  • -
Shear rate
  • -
TMP
  • -
Volumetric Throughput
  • -

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