The core of the technological infrastructure required for biopharmaceutical manufacturing is the bioreactor, used in all stages from R&D to biomanufacturing. The F0-BABY bioreactor line by BIONET is comprised of robust and user-friendly laboratory bioreactor models that can be configured for different cell expression systems like microbial cultures or cell cultures.
In the present work, the common characterization phase required in monoclonal antibody production is presented by using a CHO-S strain and BIONET’s F0-BABY 2CC bioreactor. This bioreactor has a working volume of 2L and it is equipped with the required process analytical technologies, a variable speed pump for fed batch, and an Advanced Gas Module for the control of up to 5 gas supplies. It also includes BIONET’s proprietary bioprocess software ROSITA®, for the visualization, control and data registration of all bioprocess parameters. The growth and viability of the mammalian cell culture in shake-flasks and in the bioreactor in both batch and fed batch modes were compared, as well as the production of the mAb Trastuzumab as a target product of the strain was checked. The Bionet’s F0-BABY 2CC bioreactor turned out to be a reliable and easy device to use for the growth of CHO-S cells, providing an environment that assures the lack of contamination and the most optimal cell culture conditions
Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production.Herein, we report the development of a semicontinuous process to improve the production of recombinant proteins in P.pastoris by its cultivation in a BIONET’ s F1 benchtop bioreactor of 3 L equipped with a continuous process module(CPM). Six repetitive batches were conducted in a semicontinuous process by replacing 2 / 3 of the culture every 72-96 h.The overall process in a volume of 3 L of culture yielded a protein concentration of 0.28± 0.13 mg· mL-1, […]
The majority of microbial fermentations are performed in batch or fedbatch mode, further increasing energy consumption and steps, limiting the productivity, conducting high costs. In some cases, these limitations are based on the equipment and technologies available
Tangential Flow fitlration consists on the passage of the product feed in parallel to the surface of the filtering element/membrane . The most bioprocesses use surface-active agents to reduce the foam formation, widely known as antifoams, defoamers and defoaming agents among others.
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