Production of the probiotic Lactobacillusrhamnosus GG under microaerophilicconditions in a single-use bioreactor using the Bionet Control System

Recent studies demonstrate that our microbiome can affect our health, not only with gastrointestinal diseases but also with other illnesses, such as asthma or psychological diseases (1,2). For this reason, the interest in the production of probiotics for therapeutic applications is increasing. The standards and requirements of the bioprocesses industry for high added value products, such as probiotics, are increasingly demanding, including reducing the preparation and cleaning times and the contamination risks, as well as a high level of automation and versatile and flexible control systems, to be adapted to different needs and requirements of processes. The probiotic Lactobacillus rhamnosus GG, which has raised increasing interest for its beneficial effects in human health (3-6), is a Gram-positive, non-sporeforming, facultatively anaerobic or microaerophilic bacteria. Herein, the production of this bacteria is presented, using a 3-liter single-use bioreactor (SUB) coupled with the Bionet Control System for bench-top bioreactors, and compared to a classical glass vessel to demonstrate the feasibility of using the Bionet Control System for control and monitorization of a fermentation process with a SUB. The profiles of pH and DO under microaerobic conditions with a reverse cascade were similar in both systems, with a good control temperature using only a heating blanket