Isolation of Bacillussubtilis spores by Tangential Flow Filtration using the Bionet M1 system

In downstream processing (DSP), Tangential Flow Filtration (TFF) is one of the best options to separate different molecules and/or biomass according to particle size. Bacillus sp and related products are one of the bacteria most produced by fermentation technology since it has several applications in the biotechnology industry. The main goal in DSP operations with Bacillus sp processes is the biomass or spores concentration or removal, depending on which is the target product. In this work, several filtrations using the Bionet M1 TFF system have been performed to concentrate or isolate spores using ceramic membranes of different pore sizes, and Bacillus broth produced by batch and fed-batch modes.