Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production.Herein, we report the development of a semicontinuous process to improve the production of recombinant proteins in P.pastoris by its cultivation in a BIONET’ s F1 benchtop bioreactor of 3 L equipped with a continuous process module(CPM). Six repetitive batches were […]
BIONET is taking part in the EU funded project GreenProtein. The GreenProtein project aims at producing high-added value, food-grade and fully functional proteins and other food ingredients, out of vegetable residues from the packed salad processing. Our role within the project is a crucial one, consisting on the scale-up of the process and the translation […]
The chemical cleaning of the ceramic and/or hollow fiber membranes after a filtration stage must receive the attention it deserves. Having defined an effective cleaning protocol is just as important as having optimized filtration conditions when dealing with a tangential flow filtration process in your downstream. Moreover, I would dare to say, the latter is […]
It is widely agreed that the most important quality factor of a bioreactor is its capacity to keep its inside sterile throughout the whole process and despite the number of operational human interactions that will take part during such period, including sampling and harvesting steps. Therefore, it is widely assumed that a bioreactor will do […]
«Desarrollo de un bioreactor avanzado para mejorar la productividad y flexibilidad en la fabricación de vacunas» Resumen y objetivos Desarrollar las tecnologías necesarias para crear un Bioreactor específico para la fabricación de vacunas que mejore la productividad y flexibilidad de los sistemas existentes en el mercado. Se trata de tratar de mejorar claves […]
“GREENUPGAS – Desarrollo de una tecnología de upgrading biológico para la producción de biometano en entornos agroindustriales” Resumen y objetivos El proyecto tiene como principal objetivo desarrollar la tecnología GreenUpGas o proceso biológico de purificación de biogás agroindustrial para la obtención de biometano apto para inyección en redes de gas o uso como biocarburante en […]
At BIONET, practical integration of knowledge derived from research as well as that gained from our clients’ experience happens on a continuous manner. This is vital to our evolution as a company and to the technological development and success of our products. An example of this is the effort that we deploy in adapting the […]
We always feel excited to be part of the Bio-based Industries Consortium, where engaging with bio-projects that bring lab stories to the marketplace in which they can have a societal impact BECOMES POSSIBLE! An example of this is the GREENPROTEIN project, which was granted a 5.5M euro to industrialize the isolation and purification of […]
When a continuous fermentation process is carried out, it is critical that the condition of steady-stateis reached. The main process parameters that can be used for the chemostat technique are the dilution rate, the specific growth rate and the yield of the product on the substrate
Tangential flow filtration (TFF), also known as cross flow filtration (CFF), comprises a rising technology in the last decades for the dewatering, isolation, and concentration of products of interest (e.g. biomolecules, metabolites and cells) from bioprocesses. Tangential Flow fitlration consists on the passage of the product feed in parallel to the surface of the filtering […]