Autor: Carmen

Characterization of a Bacillus amyloliquefaciens fermentation for a fed-batch strategy using the Bionet’s bBreath exhaust gas analyzer

For aerobic fermentation processes, oxygen and carbon dioxide off–gas measurementallows a better characterization and a tool for process optimization, enabling new methodsfor the control process. In the present work, a fermentation process in fed-batch modeat bench–top scale for the production of the bacteria Bacillus amyloliquefaciens wasperformed using a Bionet F0–BABY and the exhaust gas analyzer […]

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Scale-up of E. Coli culture for optimal fed-batch strategy using exponential feeding

Escherichia coli is one of the most popular expression systems for the production of recombinant proteins. In the present study, a brief protocol to apply a fed-batch mode at lab scale with E.coli and the scale-up to 30 liters was performed. The high cell density of E.coli growth was achieved using a fed-batch mode in […]

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Recombinant protein expression in Pichia pastoris in a bioreactor via semi-continuous mode

Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production. Herein, we report the development of a semi-continuous process to improve the production of recombinant proteins in P. pastoris by its cultivation in a BIONET’s F1 benchtop 3-liter bioreactor equipped with a continuous process module (CPM).Six repetitive batches were conducted […]

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Addition and Bioreactor Weight Controls

There are different fermentation modes in bioreactor systems: batch, fed-batch, and continuous mode. The batch mode is the simplest. The bioreactor is filled with the medium containing all the components that the organism requires to grow or produce determined biomolecules like a metabolite or protein. This fermentation mode has a production limitation: once the limiting […]

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5 steps into Scale-Up of Microbial Fermentation

Planning the jump into Industrial is a challenging experience that all successful bioprocesses and bioprocesists go through. With many factors having to be taken into account (at the biological, biochemical, mechanical levels among others), there is no other option than to follow a plan. What is the process information that cannot be dismissed? What process […]

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