Autor: Bionet

THE NEW CONFIGURABLE AND EXPANDABLE GAS MODULE OF OUR F0-BABY AND F1 BENCHTOP BIOREACTORS WILL UNLOCK THE POTENTIAL OF YOUR BIOPROCESS

Deciding the configuration of your bioreactor can be a challenging process, especially when looking fora benchtop system to take the first steps at this scale. For that reason, configurability and expandabilityare the main factors to consider in this situation, so the optimization of your process does not getcompromised due to a rigid and closed system. […]

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HOW TO USE THE BIONET GAS MODULE ON AN F0/F1 OPTIMALLY FOR MY PROCESS?

For the controlled production of microorganisms or living systems, gasification needs are always a criticalpart of the process regardless of its phase and its configuration can be challenging.Versatility and reliability are essential, on the one hand to find the conditions that optimize the processand on the other hand to reproduce the conditions entered from the […]

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Production in semi-continuous mode with the yeast Pichia pastoris using BIONET’s F1 bioreactor

The yeast Pichia pastoris, has been successfully used as an efficientsystem to produce heterologous proteins [1]. Herein, we report thedevelopment of a semi-continuous process to improve the productionof recombinant proteins in P. pastoris in BIONET’s F1 bench-top3-liter bioreactor, equipped with a continuous process module (CPM).Six repetitive batches were conducted in a semi-continuous processby replacing 2/3 […]

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Scale-up of a bioprocess for bio-polymers production using animal by-products.

The production model of circular economy is becoming a focus ofattention in our society. One of the solutions proposed by this modelis waste revalorization. Most of the waste revalorization processesare based in the biotransformation of these waste materials intonew valuable ones. In previous works, a fermentation process thataimed to use slaughterhouse waste as a substrate […]

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Production of the probiotic Lactobacillus rhamnosus GG under microaerophilic conditions in a single-use bioreactor using the Bionet Control System

Recent studies demonstrate that our microbiome can affect our health, notonly with gastrointestinal diseases but also with other illnesses, such as asthma or psychological diseases (1,2). For this reason, the interest in the production of probiotics for therapeutic applications is increasing. The standardsand requirements of the bioprocesses industry for high added value products,such as probiotics, […]

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Study of the effect of the substrate addition in Pichia pastoris cultures according to the evolution of exhaust gases as indicators of metabolism

The methylotrophic yeast Pichia pastoris is one of the most widely used recombinant protein expression systems, withapplications from food to the pharmaceutical industry. It is capable of growing using methanol as the only carbonsource, for which it uses alcohol oxidase (AOX) promoter, a strong inducer for recombinant protein expression. One ofthe critical aspects of methanol […]

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Isolation of Bacillus subtilis spores by Tangential Flow Filtration using the Bionet M1 system

In downstream processing (DSP), Tangential Flow Filtration (TFF) is one of the best optionsto separate different molecules and/or biomass according to particle size. Bacillus sp andrelated products are one of the bacteria most produced by fermentation technology since ithas several applications in the biotechnology industry. The main goal in DSP operations withBacillus sp processes is […]

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