Deciding the configuration of your bioreactor can be a challenging process, especially when looking fora benchtop system to take the first steps at this scale. For that reason, configurability and expandabilityare the main factors to consider in this situation, so the optimization of your process does not getcompromised due to a rigid and closed system. […]
For the controlled production of microorganisms or living systems, gasification needs are always a criticalpart of the process regardless of its phase and its configuration can be challenging.Versatility and reliability are essential, on the one hand to find the conditions that optimize the processand on the other hand to reproduce the conditions entered from the […]
The yeast Pichia pastoris, has been successfully used as an efficientsystem to produce heterologous proteins [1]. Herein, we report thedevelopment of a semi-continuous process to improve the productionof recombinant proteins in P. pastoris in BIONET’s F1 bench-top3-liter bioreactor, equipped with a continuous process module (CPM).Six repetitive batches were conducted in a semi-continuous processby replacing 2/3 […]
The production model of circular economy is becoming a focus ofattention in our society. One of the solutions proposed by this modelis waste revalorization. Most of the waste revalorization processesare based in the biotransformation of these waste materials intonew valuable ones. In previous works, a fermentation process thataimed to use slaughterhouse waste as a substrate […]
Recent studies demonstrate that our microbiome can affect our health, notonly with gastrointestinal diseases but also with other illnesses, such as asthma or psychological diseases (1,2). For this reason, the interest in the production of probiotics for therapeutic applications is increasing. The standardsand requirements of the bioprocesses industry for high added value products,such as probiotics, […]
The methylotrophic yeast Pichia pastoris is one of the most widely used recombinant protein expression systems, withapplications from food to the pharmaceutical industry. It is capable of growing using methanol as the only carbonsource, for which it uses alcohol oxidase (AOX) promoter, a strong inducer for recombinant protein expression. One ofthe critical aspects of methanol […]
In downstream processing (DSP), Tangential Flow Filtration (TFF) is one of the best optionsto separate different molecules and/or biomass according to particle size. Bacillus sp andrelated products are one of the bacteria most produced by fermentation technology since ithas several applications in the biotechnology industry. The main goal in DSP operations withBacillus sp processes is […]